![]() ![]() Climate change has been implicated as a cause of this decline not only by alteration of definitive host ranges, but due to overwinter survival of the intermediate host 14, 15, 16. tenuis has been implicated in moose ( Alces alces) declines as white-tailed deer moved into their ranges in the Midwest 2, 9, 10, 11, 12, 13. The parasite has been found in caprids, bovids, cervids, camelids, and other ungulates with variable severity of clinical disease 1, 2, 3, 4, 5, 6, 7, 8. However, aberrant migration into the brain or spinal cord is not unusual within an atypical host. Clinical signs rarely occur in WTD as the parasite primarily resides in the meninges. The larvae develop and molt into the infective å within snails or slugs which are then accidentally ingested by foraging white-tailed deer (WTD). It has an indirect life cycle in which the definitive host, white-tailed deer (Odocoileus virginianus), excretes L1 larvae in their feces that are ingested by the terrestrial gastropod intermediate host. Parelaphostrongylus tenuis is a parasitic nematode that can cause significant neurological disease and mortality in aberrant hosts both wild and domestic 1, 2, 3, 4, 5, 6, 7, 8. This method serves as a pipeline for the construction of diagnostic assays of pathogens in both human and veterinary medicine. ![]() Known negative moose sera revealed significantly lower optical density when compared to the positive samples (pā<ā0.05). These synthetic peptides were then assessed for reactivity against positive and negative moose sera and demonstrated potential use as a serological assay in diagnostic laboratories. An antigen of interest was assessed for immunogenic epitopes and subsequently synthesized into 10-mer synthetic overlapping peptides representing these regions. The proteins were analyzed using mass spectrometry and liquid chromatography to obtain amino acid sequences that were then cross-referenced to open reading frames predicted from an assembled transcriptome. tenuis organisms were affinity isolated using antibodies enriched from seropositive moose ( Alces alces). This parasite is of particular concern in both wild and domestic ungulates as it causes significant neurological signs and definitive diagnosis is only possible post-mortem, necessitating the development of serologic assays for antemortem diagnosis. Specifically, we applied these methods to a neurogenic parasitic nematode of cervids called Parelaphostrongylus tenuis. This paper outlines methods used to identify novel antigens for use in the development of serological assays. ![]()
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